Fluorescence in situ hybridization of micronuclei in binucleate fibroblasts: a protocol for cytoplasm preservation.

obtained without a phenol/chloroform extraction; however, fewer errors, greater signal strength and longer reads are obtained from the inclusion of a phenol/chloroform extraction before the butanol precipitation (Figure 1, A and C). In conclusion, this paper presents a simple dye terminator sequencing product purification protocol. This protocol is standardly used in our laboratory and provides a number of advantages over the ethanol precipitation procedure. These include higher quality sequencing data, the requirement of less time and pipetting steps, and it avoids the possibility of salt precipitation and thus, the need for a 70% ethanol wash.